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KMID : 0895720070110010069
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Journal of the Korean Society of Jungshin Science
2007 Volume.11 No. 1 p.69 ~ p.76
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Analysis of gene expression in the sleep deprived rat brain
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Kim Ji-Young
Sunu Kyung-Hee
Baik Hang-Woon
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Abstract
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Gene expression is changed by sleep and wakefulneness as well as environmental stimuli in the brain. However, the effect of sleep deprivation (SD) at the molecular level remain unexplored. It is essential to understand the restorative process occurring during sleep and the cellular mechanisms of sleep regulation. DNA microarray has been used as a powerful tool to study the gene expression in cells. To evaluate the SD related gene expression, the Sprague-Dawley rats were sleep-deprived for 96-hour. Using the cDNA chips containing a set of 5,088 sequence-verified clone, frontal lobe cerebral cortex, hypothalamus and pituitary gland for other genes whose expression may be modulated by SD were screened. The results showed that 230 transcripts were obviously modulated in the rat three brain area by 96 h sleep deprivation period. The expression levels of those genes were confirmed by realtime polymerase chain reaction (PCR). Those genes encode proteins containing immune-related proteins, DNA-binding factors, energy metabolic agents, and cell signaling proteins.
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KEYWORD
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microarray, realtime-PCR, sleep deprivation, gene expression, brain
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